HEALTH NEWS

Study Title:

Friendly Flora Inhibits Toxin Release by E. Coli

Study Abstract

AIMS: The effectiveness of four strains of Bifidobacteria against enterohemorrhagic Escherichia coli O157:H7 infection was studied using a Vero cell model.

METHODS AND RESULTS: E. coli O157 was inoculated on the Vero cell line before and after treatment with probiotic. The cytopathic effect (CPE) was evaluated during 24 h of incubation. The results indicated that Shiga toxin activity was inhibited by the probiotic. To prevent a Stx2 CPE, the probiotic needs one log more than the Stx1.

CONCLUSION: The Vero cell assay, in particular, is a good model to evaluate the effect of Bifidobacteria inhibiting bacterial attachment because of soluble substances and the competitive aspect and could be used in a variety of foods like milk and yoghurt to protect pathogen bacteria.

SIGNIFICANCE AND IMPACT OF THE STUDY: Probiotics could control pathogenic bacteria and Vero cell introduce as a model for evaluation of probiotics against pathogen bacteria.

Study Information

Tahamtan Y, Kargar M, Namdar N, Rahimian A, Hayati M, Namavari MM.
Probiotic inhibits the cytopathic effect induced by Escherichia coli O157:H7 in Vero cell line model.
Lett Appl Microbiol.
2011 May
Razi Vaccine and Serum Research Institute, Shiraz, Iran  Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran.