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Study Title:

Kinetic study of free-radical-scavenging action of biological hydroquinones (reduced forms of ubiquinone, vitamin K and tocopherol quinone) in solution.

Study Abstract

Kinetic study of free-radical-scavenging (FRS) action of eight kinds of biologically important hydroquinones (HQ's) (ubiquinol-10 (UQ10H2 1), ubiquinol-0 (UQ0H2 2), vitamin K1 HQ (VK1H2 3), vitamin K3 HQ (VK3H2 4), alpha-, beta-, gamma-tocopherol HQ's (alpha-, beta-, gamma-TQH2 5, 6, 7), and 2,3,5-trimethyl-1,4-HQ (TMQH2 8)) has been performed. The second-order rate constants, k3, for the reaction of HQ's 1-8 with substituted phenoxyl radical (PhO.) in ethanol, diethyl ether, benzene, and n-hexane have been measured with a stopped-flow spectrophotometer, as a model reaction of HQ's with unstable free radicals (LOO., LO., and HO.) in biological systems. The rate constant of UQ10H2 1 is similar to that of alpha-tocopherol in ethanol. The HQ's 3-8 showed higher reactivity than alpha-tocopherol in ethanol. Especially, the rate constants of VK1H2 3 and VK3H2 4 were found to be 31- and 21-fold larger than that of alpha-tocopherol, respectively, which has the highest reactivity among natural tocopherols. The rate constant of these HQ's increased by decreasing the polarity of solvents. The approximate order of magnitude of k3 value was (i) VK1H2 and VK3H2 > (ii) alpha-, beta-, and gamma-TQH2's and TMQH2 > (iii) alpha-tocopherol > (iv) UQ10H2 and UQ0H2 in solution. The result suggests that these biological HQ's also scavenge the active oxygen free radicals and prevent lipid peroxidation in various tissues and membranes. On the other hand, the reaction between substituted phenoxyl and biological quinones has not been observed.

Study Information

Biochim Biophys Acta. 1993 Jul 11;1157(3):313-7.

Full Study

https://www.ncbi.nlm.nih.gov/pubmed/8323961/