HEALTH NEWS

Study Title:

Effects of vitamin A deficiency on mucosal immunity and response to intestinal infection in rats.

Study Abstract

OBJECTIVE:
Vitamin A deficiency is associated with an increased incidence of infectious respiratory and alimentary tract diseases in children, and vitamin A supplementation can prevent and assist in treating these diseases. To clarify the mechanisms of these associations, we investigated the effects of vitamin A deficiency on mucosal immunity to intestinal infection in rats.
METHODS:
Specific pathogen-free Sprague-Dawley rats received a vitamin A-free diet, with (n=20) or without (n=20) vitamin A supplementation. Intestinal infection was induced by oral inoculation of salmonella in 10 rats in each group. The rats were killed 3 d after infection was induced, and we measured the number, maturation, and activation of dendritic cells; the expression of Toll-like receptors 2 and 4; mRNA level of myeloid differentiation primary response gene (88) (MyD88, pattern-recognition receptors and their adapter protein); immune cytokine production in the intestinal mucosa; and the amount of secretory immunoglobulin A in the gut.
RESULTS:
In vitamin-A deficient rats, the number of mucosal dendritic cells and the production of IL-12 markedly increased; the mucosal expressions of Toll-like receptor 2 and MyD88 were up-regulated, and secretions of interferon-γ and secretory immunoglobulin A were decreased. Infection aggravated the damage to the intestinal mucosa and lowered immunity in vitamin-A deficient rats.
CONCLUSION:
Vitamin A deficiency damaged both humoral and cellular immunity in the mucosa. Modulation of dendritic cells is likely an important mechanism through which vitamin A deficiency affects mucosal immune responses against infection.
Copyright Â© 2011 Elsevier Inc. All rights reserved.